OCEDP Research Projects

Biospecimen Preservation

OCEDP Biospecimen Preservation

Most of the experiments performed in the OCEDP laboratories rely heavily upon having access to high quality human biospecimens.  Ongoing studies for biomarker discovery and validation require human tissues (e.g. ovarian tumors, normal ovaries, and ovarian tissue with benign diseases), blood samples (sera, plasma, and white blood cells), ascites (i.e. the ascites fluid and the cell pellets), and Pap tests.  We have obtained ethical approval for all of our studies through the University of Minnesota Institutional Review Board.    

The OCEDP members are well aware of the detrimental effects that pre-analytical variables can have on research projects.  For example, if biospecimens are not handled properly when they are removed from the patient and then processed immediately, they will not be suitable for doing experiments and the data that is generated from these biospecimens will not be reliable.  The entire team of surgeons, clinicians, nurses, and clinical trial coordinators have played an important role in ensuring that the work flow of biospecimens in the clinics and operating rooms is optimized for patient care and biospecimen quality.

For this reason, Dr. Amy Skubitz established the Tissue Procurement Facility (TPF) in 1995 from the ground up as a core facility for the University of Minnesota’s Masonic Cancer Center.   She served as the Director or Assistant Director for almost two decades.  During this time, the Tissue Procurement Facility became part of the Academic Health Center’s BioNet, and it is currently a key component of the University of Minnesota’s Clinical and Translational Science Institute.  A main mission of the BioNet TPF is to consent patients prior to surgery and then procure and process tissue and blood samples for researchers, while maintaining patient confidentiality.

Dr. Skubitz has been a member of the International Society for Biological and Environmental Repositories (ISBER) since 1999.  She has served as a member of ISBER’s Education and Training Committee for 6 years; serving as an Associate Editor for three editions of the “Best Practices for Repositories”.  She is also a member of ISBER’s Biospecimen Science Working Group.  Dr. Skubitz and members of the OCEDP have contributed to the publication of manuscripts that emphasize the need for standard operating procedures, quality assurance, and quality controls in all aspects of biobanking.

Articles

Isothermal vitrification methodology development for non-cryogenic storage of archival human sera.

Less RBoylan KLSkubitz APAksan A.

Biorepositories worldwide collect human serum samples and store them for future research. Currently, hundreds of biorepositories across the world store human serum samples in refrigerators, freezers, or liquid nitrogen without following any specific cryopreservation protocol. This method of storage is both expensive and potentially detrimental to the biospecimens.

View full article here.


State of the art in preservation of fluid biospecimens.

Hubel AAksan ASkubitz APWendt CZhong X.

Abstract: Fluid biospecimens (blood, serum, urine, saliva, cerebrospinal fluid and bronchial lavage fluid) contain not only cells and subcellular components, but also proteins, enzymes, lipids, metabolites, and peptides, which are utilized as biomarkers. Availability of high-quality biospecimens is a requirement for biomarker discovery. The quality of the biospecimens depends upon preanalytical variables (ie, collection and processing techniques, freeze/thaw stability, and storage stability), which account for >60%-90% of the diagnostic errors. Currently, millions of fluid biospecimens are stored in hundreds of biorepositories across the nation, and tens of thousands of new biospecimens are added to the pool daily. Specimen stabilization is imperative, because fluid biospecimens degrade quickly when kept untreated at room temperature. Achieving a high-quality fluid biospecimen requires understanding the effects of storage processing parameters (eg, freezing and thawing as well as cryo-/lyoprotectant additives) and storage conditions on biomarkers contained within the biospecimens. In this article, we will discuss the main issues related to the stabilization of specific biofluids by reviewing (a) the current preservation and storage practices applied in biobanks/biorepositories and (b) the sensitivity of certain biomarkers to current storage techniques.

 

ISBER Best Practices

2012 best practices for repositories collectionstorageretrieval, and distribution of biological materials for research international society for biological and environmental repositories.

Introduction: The availability of high quality biological and environmental specimens for research purposes requires the development of standardized methods for collection, long-term storageretrieval and distribution of specimens that will enable their future use.

View full article here


Development of the ISBER Best Practices for Repositories: Collection, Storage, Retrieval and Distribution of Biological Materials for Research.

Campbell LDBetsou FGarcia DLGiri JGPitt KEPugh RSSexton KCSkubitz APSomiari SB.

View information here

ISBER Working Group

Human biospecimen researchexperimental protocol and quality control tools.

Betsou FBarnes RBurke TCoppola DDesouza YEliason JGlazer BHorsfall DKleeberger CLehmann SPrasad ASkubitz ASomiari SGunter E[International Society for Biological and Environmental Repositories (ISBER) Working Group on Biospecimen Science].

View the full article here


Standard preanalytical coding for biospecimens: review and implementation of the Sample PREanalytical Code (SPREC).

Lehmann SGuadagni FMoore HAshton GBarnes MBenson EClements JKoppandi ICoppola DDemiroglu SYDeSouza YDe Wilde ADuker JEliason JGlazer BHarding KJeon JPKessler JKokkat TNanni UShea KSkubitz ASomiari STybring GGunter EBetsou FInternational Society for Biological and Environmental Repositories (ISBER) Working Group on Biospecimen Science.

Abstract: The first version of the Standard PREanalytical Code (SPREC) was developed in 2009 by the International Society for Biological and Environmental Repositories (ISBER) Biospecimen Science Working Group to facilitate documentation and communication of the most important preanalytical quality parameters of different types of biospecimens used for research. This same Working Group has now updated the SPREC to version 2.0, presented here, so that it contains more options to allow for recent technological developments. Existing elements have been fine tuned. An interface to the Biospecimen Reporting for Improved Study Quality (BRISQ) has been defined, and informatics solutions for SPREC implementation have been developed. A glossary with SPREC-related definitions has also been added.

View full article here


Identification of evidence-based biospecimen quality-control tools: a report of the International Society for Biological and Environmental Repositories (ISBER) Biospecimen Science Working Group.

Betsou FGunter EClements JDeSouza YGoddard KAGuadagni FYan WSkubitz ASomiari SYeadon TChuaqui R.

AbstractControl of biospecimen quality that is linked to processing is one of the goals of biospecimen science. Consensus is lacking, however, regarding optimal sample quality-control (QC) tools (ie, markers and assays). The aim of this review was to identify QC tools, both for fluid and solid-tissue samples, based on a comprehensive and critical literature review. The most readily applicable tools are those with a known threshold for the preanalytical variation and a known reference range for the QC analyte. Only a few meaningful markers were identified that meet these criteria, such as CD40L for assessing serum exposure at high temperatures and VEGF for assessing serum freeze-thawing. To fully assess biospecimen quality, multiple QC markers are needed. Here we present the most promising biospecimen QC tools that were identified.

View the full article here.

Research Projects

Meet the Team

Facilities at UMN

Facilities at UMN

Research Funding Support

Research Funding Support

Funding for the OCEDP has been provided by:

National Institutes of Health / National Cancer Institute

Department of Defense Congressionally Directed Medical Research Programs

Minnesota Ovarian Cancer Alliance 

Cancurables Foundation

Charlene's Light 

University of Minnesota

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Contact Us

Contact Us

Contact the Director:

Amy P.N. Skubitz, Ph.D.
Professor, Department of Laboratory Medicine and Pathology
Adjunct Professor, Dept Obstetrics, Gynecology, and Women's Health
University of Minnesota
MMC 395
420 Delaware Street, S.E.
Minneapolis, MN  55455
skubi002@umn.edu

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